{"product_id":"bence-jones-test-piastra-a-doppia-immunodiffusione-20x2-test","title":"BENCE JONES TEST Double immunodiffusion plate 20x2 tests","description":"\u003cp\u003eThe \u003cstrong\u003eBENCE JONES TEST\u003c\/strong\u003e is an innovative diagnostic kit designed for the determination of kappa and lambda light chains (Bence Jones proteins) in urine, using the double immunodiffusion method. This test is essential for the diagnosis of conditions such as multiple myeloma and other disorders associated with high production of these proteins.\u003c\/p\u003e\u003ch2\u003eProduct Features\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\u003cstrong\u003eComposition:\u003c\/strong\u003e Each plate of the BENCE JONES TEST consists of a layer of agarose gel, with sodium azide (1 g\/l) as preservative.\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e Product exclusively for in vitro diagnostic use.\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at temperatures between +2° and +8°C. Usable until the expiration date indicated on the label.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eTest Principle\u003c\/h2\u003e\u003cp\u003eThe test is based on the \u003cstrong\u003eOuchterlony technique\u003c\/strong\u003e, which allows the determination of kappa and lambda light chains through immunodiffusion in agarose gel. The antigens and antisera, placed in different wells of the gel, diffuse and react forming a visible precipitation band, indicating the presence of these proteins in urine.\u003c\/p\u003e\u003ch2\u003eProcedure\u003c\/h2\u003e\u003col\u003e\u003cli\u003eOpen the plate and leave it at room temperature for about 5 minutes to allow any water droplets to evaporate.\u003c\/li\u003e\u003cli\u003eLoad 40µl of the urine sample into the central well.\u003c\/li\u003e\u003cli\u003eAdd 10µl of kappa antiserum into the right side well and 10µl of lambda antiserum into the left side well.\u003c\/li\u003e\u003cli\u003eAllow to absorb for 20 minutes, then close the plate with the lid and turn it upside down.\u003c\/li\u003e\u003cli\u003ePlace the plate back in its pouch and leave it at 37°C for 9-15 hours for diffusion.\u003c\/li\u003e\u003c\/ol\u003e\u003ch2\u003eResult Evaluation\u003c\/h2\u003e\u003cp\u003eResult evaluation is simple and straightforward:\u003c\/p\u003e\u003cul\u003e\u003cli\u003e\u003cstrong\u003eAbsence of bands:\u003c\/strong\u003e Excludes the presence of kappa or lambda light chains in urine.\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003ePresence of bands:\u003c\/strong\u003e Indicates the presence of light chains; the position of the band allows estimation of the sample concentration.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eThe sensitivity of the method is approximately 2.8 mg\/dl for K chains and 2.1 mg\/dl for lambda chains.\u003c\/p\u003e\u003ch2\u003eKit Contents\u003c\/h2\u003e\u003cp\u003eEach kit includes:\u003c\/p\u003e\u003cul\u003e\u003cli\u003e20 plates with agarose gel\u003c\/li\u003e\u003cli\u003e1 vial of Anti h-Ig\/kappa chains x 400 µl\u003c\/li\u003e\u003cli\u003e1 vial of Anti h-Ig\/lambda chains x 400 µl\u003c\/li\u003e\u003cli\u003e1 vial of positive control type kappa and lambda x 100µl\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eWarnings and Precautions\u003c\/h2\u003e\u003cp\u003eIt is recommended not to ingest the product and to avoid contact with skin and mucous membranes, especially when using in vitro diagnostics containing sodium azide.\u003c\/p\u003e\u003cp\u003eThe \u003cstrong\u003eBENCE JONES TEST\u003c\/strong\u003e is an essential tool for healthcare professionals, providing rapid and reliable results for the diagnosis of critical clinical conditions.\u003c\/p\u003e","brand":"LIOFILCHEM","offers":[{"title":"Default Title","offer_id":57603139207550,"sku":"LF93201","price":173.32,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0959\/4873\/4846\/files\/LF93201_--_000_30120.jpg?v=1782257739","url":"https:\/\/www.securlab.it\/en\/products\/bence-jones-test-double-immunodiffusion-plate-20x2-tests","provider":"Securlab","version":"1.0","type":"link"}