{"product_id":"chromatic-mh-e-un-terreno-cromogenico-base-mueller-hinton-20-piastre","title":"Chromatic™ MH is a chromogenic medium (Mueller Hinton base) 20 plates","description":"\u003ch3 data-end=\"196\" data-start=\"101\"\u003eChromatic™ MH – Chromogenic Mueller Hinton for microbial differentiation and antibiogram\u003c\/h3\u003e\u003cp data-end=\"477\" data-start=\"198\"\u003e\u003cstrong data-end=\"213\" data-start=\"198\"\u003eDescription\u003c\/strong\u003e\u003cbr data-end=\"216\" data-start=\"213\"\/\u003eChromatic™ MH is a Mueller Hinton-based chromogenic medium used for microbial differentiation together with antibiogram testing for various microorganisms directly from clinical samples. The antibiogram is optimized for Gram-negative bacteria.\u003c\/p\u003e\u003ch3 data-end=\"505\" data-start=\"479\"\u003eTypical formula (g\/l)\u003c\/h3\u003e\u003cp data-end=\"644\" data-start=\"506\"\u003eMeat extract 2.0\u003cbr data-end=\"530\" data-start=\"527\"\/\u003eAcid casein hydrolysate 17.5\u003cbr data-end=\"566\" data-start=\"563\"\/\u003eStarch 1.5\u003cbr data-end=\"578\" data-start=\"575\"\/\u003eChromogenic mixture 0.5\u003cbr data-end=\"604\" data-start=\"601\"\/\u003eAgar 17.0\u003cbr data-end=\"616\" data-start=\"613\"\/\u003eFinal pH 7.3 ± 0.2 at 25°C\u003c\/p\u003e\u003ch3 data-end=\"672\" data-start=\"646\"\u003ePrinciple of the method\u003c\/h3\u003e\u003cp data-end=\"1018\" data-start=\"673\"\u003eThe acid casein hydrolysate and meat extract provide amino acids, nitrogen, carbon, minerals, vitamins, and other essential nutrients for microbial growth. Starch acts as a protective colloid against toxic molecules and, through its hydrolysis in the autoclave, provides a small amount of glucose as an energy source.\u003c\/p\u003e\u003cp data-end=\"1177\" data-start=\"1020\"\u003eThe chromogenic mixture makes it possible to identify microorganisms based on the color and morphology of the colonies. Agar acts as a solidifying agent.\u003c\/p\u003e\u003cp data-end=\"1524\" data-start=\"1179\"\u003eThe Kirby-Bauer method is based on the diffusion of the antimicrobial through the agar. Each paper disc contains a specific concentration of antimicrobial agent, which inhibits the growth of the microorganism by forming a halo around the disc. The diameter of the inhibition halo is correlated with the minimum inhibitory concentration (MIC).\u003c\/p\u003e\u003cp data-end=\"1925\" data-start=\"1526\"\u003eThe Gradient Strip method is a quantitative analysis for determining the MIC of an antimicrobial agent and for identifying resistance mechanisms. The strip contains an exponential gradient of the antimicrobial agent and is placed on the surface of the inoculated plate. The MIC value is read where the edge of the inhibition ellipse intersects the strip.\u003c\/p\u003e\u003cp data-end=\"2047\" data-start=\"1927\"\u003eChromatic™ MH Agar is formulated with low levels of thymine and thymidine and controlled levels of calcium and magnesium ions.\u003c\/p\u003e\u003ch3 data-end=\"2067\" data-start=\"2049\"\u003ePreparation\u003c\/h3\u003e\u003cp data-end=\"2364\" data-start=\"2068\"\u003eFor the dehydrated medium, suspend 38.5 g of powder in 1 liter of sterile distilled or deionized water, mix well and heat until completely dissolved. Sterilize in an autoclave at 121°C for 15 minutes, cool to 45-50°C and dispense into Petri dishes under aseptic conditions.\u003c\/p\u003e\u003ch3 data-end=\"2390\" data-start=\"2366\"\u003eTest procedure\u003c\/h3\u003e\u003cp data-end=\"2739\" data-start=\"2391\"\u003eInoculate the plate by streaking 10 µl of sample directly onto the agar surface. If necessary, dilute the sample in saline solution and mix with a vortex before plating.\u003cbr data-end=\"2588\" data-start=\"2585\"\/\u003eApply the antimicrobial discs or MIC Test Strips onto the surface of the inoculated plate.\u003cbr data-end=\"2685\" data-start=\"2682\"\/\u003eIncubate at 37°C for 18-24 hours in aerobic atmosphere.\u003c\/p\u003e\u003ch3 data-end=\"2776\" data-start=\"2741\"\u003eInterpretation of results\u003c\/h3\u003e\u003cp data-end=\"2884\" data-start=\"2777\"\u003eAfter incubation, observe the color of the colonies and interpret the results according to the ID table.\u003c\/p\u003e\u003cp data-end=\"3126\" data-start=\"2886\"\u003eMicroorganisms and typical colony color:\u003cbr data-end=\"2933\" data-start=\"2930\"\/\u003eE. coli pink-reddish-mauve\u003cbr data-end=\"2964\" data-start=\"2961\"\/\u003eKlebsiella spp, Enterobacter spp, Serratia spp green-blue\u003cbr data-end=\"3023\" data-start=\"3020\"\/\u003eProteus spp brown\u003cbr data-end=\"3045\" data-start=\"3042\"\/\u003ePseudomonas spp yellowish-green\u003cbr data-end=\"3080\" data-start=\"3077\"\/\u003eS. aureus cream\u003cbr data-end=\"3098\" data-start=\"3095\"\/\u003eE. faecalis green-turquoise\u003c\/p\u003e\u003cp data-end=\"3431\" data-start=\"3128\"\u003eFor the antibiogram, measure the diameter of the inhibition zone around the disc or read the MIC value. Interpret the inhibition zone diameters or MIC values according to current standards and classify the microorganism as susceptible, intermediate, or resistant to the tested agents.\u003c\/p\u003e\u003ch3 data-end=\"3462\" data-start=\"3433\"\u003eAppearance and storage\u003c\/h3\u003e\u003cp data-end=\"3569\" data-start=\"3463\"\u003eDehydrated medium: fine, dry, homogeneous, beige\u003cbr data-end=\"3518\" data-start=\"3515\"\/\u003ePrepared medium: amber, slightly opalescent\u003c\/p\u003e\u003cp data-end=\"3894\" data-start=\"3571\"\u003eThe powder is highly hygroscopic and must be stored at 10-30°C in a dry environment, in the original tightly closed container. Ready-to-use plates must be stored at 2-8°C protected from light. Do not use the product after the expiry date or if it shows signs of contamination or deterioration.\u003c\/p\u003e\u003ch3 data-end=\"3908\" data-start=\"3896\"\u003eShelf life\u003c\/h3\u003e\u003cp data-end=\"3971\" data-start=\"3909\"\u003eDehydrated medium: 4 years\u003cbr data-end=\"3939\" data-start=\"3936\"\/\u003eReady-to-use plates: 4 months\u003c\/p\u003e\u003cp data-end=\"4075\" data-is-last-node=\"\" data-start=\"3973\"\u003eBuy Chromatic™ MH now on Securlab.it for accurate and reliable microbiological identification.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e","brand":"LIOFILCHEM","offers":[{"title":"Default Title","offer_id":57603169059198,"sku":"LF11618","price":32.57,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0959\/4873\/4846\/files\/LF11618_--_000_44691.jpg?v=1782257686","url":"https:\/\/www.securlab.it\/en\/products\/chromatic-mh-is-a-chromogenic-medium-mueller-hinton-base-20-plates","provider":"Securlab","version":"1.0","type":"link"}