{"product_id":"terreno-cromogenico-per-la-ricerca-ed-il-conteggio-di-escherichia-coli-20-piastre-da-90-mm","title":"Chromogenic medium for the detection and enumeration of Escherichia coli 20 plates of 90 mm","description":"\u003cp\u003e\u003cstrong\u003eChromaticTM Coli Coliform Chromogenic Medium\u003c\/strong\u003e is a high-quality product designed for the detection and enumeration of \u003cstrong\u003eEscherichia coli\u003c\/strong\u003e and coliform bacteria in food and water samples. Thanks to its selective formulation, this medium makes it possible to effectively identify the microorganisms present, facilitating microbiological analysis in laboratories and quality control facilities.\u003c\/p\u003e\u003ch2\u003eProduct Features\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\u003cstrong\u003eFormat:\u003c\/strong\u003e 20 plates of 90 mm\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eType of medium:\u003c\/strong\u003e Chromogenic and selective\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e Microbiological analysis of food and water\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eMedium Composition\u003c\/h2\u003e\u003cp\u003eThe typical formulation of the medium is composed of:\u003c\/p\u003e\u003cul\u003e\u003cli\u003e\u003cstrong\u003ePeptone:\u003c\/strong\u003e 15.0 g\/l - source of amino acids and essential nutrients\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eYeast Extract:\u003c\/strong\u003e 3.0 g\/l - rich in B vitamins\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eSodium Chloride:\u003c\/strong\u003e 5.0 g\/l - maintains osmotic balance\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eChromogenic and Selective Mixture:\u003c\/strong\u003e 1.7 g\/l - for colorimetric identification of microorganisms\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eAgar:\u003c\/strong\u003e 15.0 g\/l - solidifying agent\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eFinal pH:\u003c\/strong\u003e 7.2 ± 0.2 at 25°C\u003c\/p\u003e\u003ch2\u003eMethod Principle\u003c\/h2\u003e\u003cp\u003eThe \u003cstrong\u003eChromogenic Medium\u003c\/strong\u003e works thanks to a selective inhibition mechanism, which allows the growth of \u003cstrong\u003eE. coli\u003c\/strong\u003e and coliform bacteria while hindering the proliferation of most Gram-positive bacteria. The colonies of \u003cstrong\u003eE. coli\u003c\/strong\u003e appear green, while the other coliform bacteria take on a mauve coloration.\u003c\/p\u003e\u003ch2\u003eMedium Preparation\u003c\/h2\u003e\u003cp\u003ePreparing the medium is simple and requires the following steps:\u003c\/p\u003e\u003cul\u003e\u003cli\u003eFor the dehydrated medium: suspend 39.7 g of powder in 1 liter of sterile distilled or deionized water.\u003c\/li\u003e\u003cli\u003eMix well and heat until completely dissolved (do not autoclave).\u003c\/li\u003e\u003cli\u003eFor the medium in bottles: dissolve the contents in a water bath at 100°C until completely dissolved.\u003c\/li\u003e\u003cli\u003ePour into Petri dishes under aseptic conditions after cooling to 45-50°C.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eTest Procedure\u003c\/h2\u003e\u003cp\u003eTo test samples, follow these instructions:\u003c\/p\u003e\u003cul\u003e\u003cli\u003eInoculate solid samples directly onto the surface of the medium.\u003c\/li\u003e\u003cli\u003eInoculate liquid samples by spreading or using membrane filtration technique.\u003c\/li\u003e\u003cli\u003eIncubate at 35 ± 2°C for 18-24 hours in an aerobic atmosphere.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eInterpretation of Results\u003c\/h2\u003e\u003cp\u003eAfter incubation, observe the color and morphology of the colonies. The results can be interpreted as follows:\u003c\/p\u003e\u003ctable\u003e\u003ctr\u003e\u003cth\u003eMicroorganism\u003c\/th\u003e\u003cth\u003eTypical colony color\u003c\/th\u003e\u003c\/tr\u003e\u003ctr\u003e\u003ctd\u003eE. coli*\u003c\/td\u003e\u003ctd\u003eGreen\u003c\/td\u003e\u003c\/tr\u003e\u003ctr\u003e\u003ctd\u003eOther coliform bacteria\u003c\/td\u003e\u003ctd\u003eMauve\u003c\/td\u003e\u003c\/tr\u003e\u003ctr\u003e\u003ctd\u003eOther bacteria (if not inhibited)\u003c\/td\u003e\u003ctd\u003eColorless\u003c\/td\u003e\u003c\/tr\u003e\u003c\/table\u003e\u003cp\u003e*Note: \u003cstrong\u003eE. coli\u003c\/strong\u003e ß-glucuronidase-negative strains, such as \u003cstrong\u003eE. coli O157\u003c\/strong\u003e, are colorless on this medium.\u003c\/p\u003e\u003ch2\u003eAdvantages of Use\u003c\/h2\u003e\u003cul\u003e\u003cli\u003eRapid and accurate identification of \u003cstrong\u003eE. coli\u003c\/strong\u003e and coliform bacteria.\u003c\/li\u003e\u003cli\u003eEase of preparation and use.\u003c\/li\u003e\u003cli\u003eSuitable for analyses in food and quality control laboratories.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eThe \u003cstrong\u003eChromaticTM Coli Coliform Chromogenic Medium\u003c\/strong\u003e is an indispensable ally for ensuring food safety and water quality, providing reliable and timely results.\u003c\/p\u003e","brand":"LIOFILCHEM","offers":[{"title":"Default Title","offer_id":57603104899454,"sku":"LF11613","price":26.16,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0959\/4873\/4846\/files\/LF11613_--_000_29678.jpg?v=1783430775","url":"https:\/\/www.securlab.it\/en\/products\/chromogenic-medium-for-the-detection-and-enumeration-of-escherichia-coli-20-plates-of-90-mm","provider":"Securlab","version":"1.0","type":"link"}