{"product_id":"colorante-per-microscopia-dif-stain-3x250-mi","title":"DIF-STAIN microscopy stain 3x250 mI","description":"\u003cp\u003eDiscover DIF-STAIN microscopy stain, an innovative and highly effective solution for the differential staining of blood formed elements and other biological samples. This stain is a variant of the classic contrast stain Methylene Blue Thiazine and Eosin (Romanowsky), designed to provide fast and reliable results in the laboratory.\u003c\/p\u003e\u003ch2\u003eProduct Features\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\u003cstrong\u003eFormat:\u003c\/strong\u003e 3 bottles of 250 mL each\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eType:\u003c\/strong\u003e Microscopy stain\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eApplications:\u003c\/strong\u003e Blood smears, bone marrow, urinary sediments, sputum, epithelial tissue samples\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eAdvantages of DIF-STAIN Staining\u003c\/h2\u003e\u003cp\u003eDIF-STAIN staining offers numerous advantages for medical and research professionals:\u003c\/p\u003e\u003cul\u003e\u003cli\u003e\u003cstrong\u003eSpeed:\u003c\/strong\u003e Fast method for differential staining, ideal for laboratories with high sample volumes.\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eVersatility:\u003c\/strong\u003e Usable on various types of preparations, including vaginal smears and cytocentrifuged samples.\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eResult Quality:\u003c\/strong\u003e Provides a color pattern comparable to the traditional May Grünwald – Giemsa method.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eComposition of DIF-STAIN\u003c\/h2\u003e\u003cp\u003eThe DIF-STAIN stain is composed of:\u003c\/p\u003e\u003col\u003e\u003cli\u003e\u003cstrong\u003eFixative:\u003c\/strong\u003e Methyl Alcohol solution (colorless or green-colored)\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eStain A:\u003c\/strong\u003e Eosin Y in phosphate buffer\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eStain B:\u003c\/strong\u003e Thiazine stain in phosphate buffer\u003c\/li\u003e\u003c\/ol\u003e\u003ch2\u003eStaining Protocol\u003c\/h2\u003e\u003ch3\u003eProtocol for Smears\u003c\/h3\u003e\u003col\u003e\u003cli\u003eAir-dry the smear and fix it in DIF-STAIN Fixative.\u003c\/li\u003e\u003cli\u003ePour off the excess Fixative.\u003c\/li\u003e\u003cli\u003eImmerse the slide 5 times for 1 second in solution A, removing excess liquid after each immersion.\u003c\/li\u003e\u003cli\u003eRepeat the previous step with solution B.\u003c\/li\u003e\u003cli\u003eShake the smear to mix the stains.\u003c\/li\u003e\u003cli\u003eWash in tap water and rinse in distilled water or Weise buffer pH 7.2.\u003c\/li\u003e\u003cli\u003eAllow the slide to dry in a vertical position.\u003c\/li\u003e\u003c\/ol\u003e\u003ch3\u003eProtocol for Paraffin Sections\u003c\/h3\u003e\u003col\u003e\u003cli\u003eBring the sections to water.\u003c\/li\u003e\u003cli\u003eStain with solution A for 30 seconds.\u003c\/li\u003e\u003cli\u003eStain with solution B for 30 seconds.\u003c\/li\u003e\u003cli\u003eQuickly rinse in distilled water.\u003c\/li\u003e\u003cli\u003eDehydrate and clear.\u003c\/li\u003e\u003cli\u003eMount the slide and observe under the microscope.\u003c\/li\u003e\u003c\/ol\u003e\u003ch2\u003eInterpretation of Results\u003c\/h2\u003e\u003cp\u003eDIF-STAIN staining allows for a detailed color pattern, useful for interpreting samples. Results can be compared with those of the classic May Grünwald – Giemsa method:\u003c\/p\u003e\u003cul\u003e\u003cli\u003e\u003cstrong\u003eEosinophilic Granules:\u003c\/strong\u003e Reddish-brown\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eBasophilic Granules:\u003c\/strong\u003e Blue\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eNuclei:\u003c\/strong\u003e Blue-violet\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eNuclei of Blood Parasites and Protozoa:\u003c\/strong\u003e Bright red\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eNeutrophilic Granules:\u003c\/strong\u003e Red-violet\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eLymphocyte Protoplasm:\u003c\/strong\u003e Blue with possible purple-red granules\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003ePlatelets:\u003c\/strong\u003e Blue with violet central body\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eErythrocytes:\u003c\/strong\u003e Pale pink\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eMicroorganisms:\u003c\/strong\u003e Blue-violet\u003c\/li\u003e\u003cli\u003e\u003cstrong\u003eSpermatozoa:\u003c\/strong\u003e Dark blue\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eUse DIF-STAIN stain to obtain high-quality and precise results in your microscopic analysis. Perfect for clinical and research laboratories, this product is an excellent choice for those seeking efficiency and reliability.\u003c\/p\u003e","brand":"KALTEK","offers":[{"title":"Default Title","offer_id":57603038413182,"sku":"1526","price":62.78,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0959\/4873\/4846\/files\/1526_--_000_38182.jpg?v=1782252376","url":"https:\/\/www.securlab.it\/en\/products\/dif-stain-microscopy-stain-3x250-mi","provider":"Securlab","version":"1.0","type":"link"}