Discover the DIF-STAIN microscopy dye, an innovative and highly effective solution for the differential staining of blood elements and other biological samples. This staining is a variant of the classic contrast staining of Methylene Blue Thiazine and Eosin (Romanowsky), designed to provide quick and reliable results in the laboratory.

Product Features

• Format: 3 bottles of 250 mL each


• Type: Microscopy dye


• Applications: Blood smears, bone marrow, urinary sediments, sputum, epithelial tissue samples

Advantages of DIF-STAIN Staining

The DIF-STAIN staining offers numerous advantages for medical and research professionals:

• Speed: Fast method for differential staining, ideal for laboratories with high sample volumes.


• Versatility: Usable on various types of preparations, including vaginal smears and cytocentrifuges.


• Quality of Results: Provides a color scheme comparable to the traditional May Grünwald – Giemsa method.

DIF-STAIN Composition

The DIF-STAIN dye consists of:

1. Fixative: Solution of Methyl Alcohol (colorless or colored green)


2. Dye A: Eosin Y in phosphate buffer


3. Dye B: Thiazine dye in phosphate buffer

Staining Protocol

Protocol for Smears

1. Air dry the smear and fix it in the DIF-STAIN Fixative.


2. Pour off excess Fixative.


3. Immerse the slide 5 times for 1 second in Solution A, removing excess liquid after each immersion.


4. Repeat the previous step with Solution B.


5. Shake the smear to mix the dyes.


6. Wash in spring water and rinse in distilled water or Weise buffer pH 7.2.


7. Allow the slide to dry in an upright position.

Protocol for Paraffin Sections

1. Bring the sections to water.


2. Stain with Solution A for 30 seconds.


3. Stain with Solution B for 30 seconds.


4. Quickly rinse in distilled water.


5. Dehydrate and clarify.


6. Mount the slide and observe under the microscope.

Interpretation of Results

The DIF-STAIN staining allows for a detailed color scheme, useful for interpreting samples. The results can be compared with those of the classic May Grünwald – Giemsa method:

• Eosinophilic Granules: Reddish-brown


• Basophilic Granules: Blue


• Nuclei: Blue-violet


• Nuclei of Blood Parasites and Protozoa: Bright red


• Neutrophilic Granules: Red-violet


• Lymphocyte Protoplasm: Blue with possible reddish-purple granules


• Platelets: Blue with a violet central body


• Erythrocytes: Pale pink


• Microorganisms: Blue-violet


• Spermatozoa: Dark blue

Use the DIF-STAIN dye to achieve high-quality and precise results in your microscopic analysis. Perfect for clinical and research laboratories, this product represents an excellent choice for those seeking efficiency and reliability.