Mycoplasma Transport Broth is a highly specialized liquid transport medium designed for the enrichment and recovery of mycoplasmas and other pathogenic microorganisms. This product is essential for clinical and research laboratories involved in the diagnosis of sexually transmitted infections and other related conditions. Thanks to its unique formulation, Mycoplasma Transport Broth ensures the preservation of microorganism viability during transport, delivering reliable and timely results.

Main Features

• Nutritional Composition: Contains selected ingredients such as tryptone, soy peptone, and glucose, which promote microbial growth.


• Selective Agent: The addition of thallium acetate makes it possible to isolate mycoplasmas while maintaining the viability of sensitive organisms.


• Ease of Use: Simple inoculation and incubation procedure, ideal for laboratories with diverse diagnostic needs.

Clinical Applications

Mycoplasma Transport Broth is particularly useful for:

• Collection and transport of clinical samples such as vaginal, urethral, and seminal fluid swabs.


• Isolation of mycoplasmas from samples from animals or the environment.


• Culture of pathogenic microorganisms such as Trichomonas vaginalis, Gardnerella vaginalis, and Candida spp..

Use Procedure

To ensure optimal results, follow these steps:

1. Bring Mycoplasma Transport Broth to room temperature before use.


2. Inoculate the sample into the broth and send it to the laboratory for prompt analysis.


3. Refrigerate the sample to reduce the growth of contaminants, or keep it at room temperature to improve the recovery of some organisms.


4. Incubate at 36 ± 1°C for 36-72 hours.


5. Perform subcultures on appropriate solid media for final analysis.

Quality Control and Safety

Mycoplasma Transport Broth undergoes rigorous quality testing, using standard organisms such as Mycoplasma hominis ATCC® 23114. It is important to note that:

• The product is not classified as hazardous under current regulations.


• It is intended exclusively for in vitro diagnostic use by qualified personnel.

Storage Conditions

To maintain product effectiveness, follow these guidelines:

• Store at 2-8°C protected from light.


• Stability has also been demonstrated at temperatures up to 25°C for 4 days or at 39°C for 48 hours without loss of effectiveness.

Factors That May Influence Results

It is essential to consider that variables such as sample volume, timing, and collection conditions may affect the reliability of the results. Therefore, it is essential to follow standardized procedures to ensure diagnostic accuracy.

In conclusion, Mycoplasma Transport Broth is an essential component for laboratories involved in the diagnosis of sexually transmitted infections, offering an effective medium for the transport and culture of urogenital pathogens.