Sabouraud Dextrose Agar is a fundamental culture medium for microbiologists and analytical laboratories, specifically designed for the growth and enumeration of yeasts and molds from a variety of materials. This product complies with EN ISO 11133 and USP/EP/JP standards, thus ensuring reliable and reproducible results for microbiological analyses.

Features of Sabouraud Dextrose Agar

• Format: Pack of 20 90 mm plates, practical and ready to use.


• Type of medium: Non-selective, ideal for the growth of pathogenic and non-pathogenic fungi.


• Applications: Used for clinical and non-clinical samples, environmental monitoring, and recovery of microorganisms.


• Compliance: Meets EN ISO 11133, USP, EP, and JP regulations for microbiological examination.

Medium Composition

The typical formulation of Sabouraud Dextrose Agar is as follows:

• Pancreatic Digest of Casein: 5.0 g/l


• Peptic Digest of Animal Tissues: 5.0 g/l


• Dextrose: 40.0 g/l


• Agar: 15.0 g/l


• Final pH: 5.6 ± 0.2 at 25°C

Principle of the Method

Sabouraud Dextrose Agar provides an ideal nutrient-rich environment for the growth of microorganisms thanks to the presence of:

• Amino acids and nutrients: provided by the pancreatic digest of casein and the peptic digest of animal tissues.


• Energy source: dextrose, which promotes rapid fungal growth.


• Solidifying agent: agar, which allows easy handling and isolation of microorganisms.

The combination of a high dextrose concentration and an acidic pH makes this medium particularly selective for fungi. Chloramphenicol can be added to improve bacterial inhibition and optimize the recovery of dermatophytes.

Use and Benefits

Sabouraud Dextrose Agar is particularly useful in contexts such as:

• Clinical microbiology laboratories


• Quality control in food and pharmaceutical industries


• Environmental monitoring in sensitive areas

The plates are gamma-sterilized and packaged in triple vacuum-sealed wrapping, ensuring maximum safety and product integrity, making them ideal for use in microbiologically controlled environments such as isolators and clean rooms.

Conclusion

In summary, Sabouraud Dextrose Agar is an essential product for those working in the field of microbiology, offering a highly effective culture medium that complies with international standards. Choose our Sabouraud Dextrose Agar to obtain reliable and accurate results in your microbiological analyses.