Selenite Broth is a highly specialized liquid medium, designed for the selective enrichment of Salmonella spp. and some species of Shigella. This product is essential for microbiology laboratories and for the analysis of clinical and non-clinical samples, following the guidelines established by the American Public Health Association (APHA).

Characteristics of Selenite Broth

Prepared according to the original Leifson formula, Selenite Broth is recommended for food testing and clinical diagnostics. Its unique composition promotes the growth of pathogenic bacteria while inhibiting the proliferation of other unwanted microorganisms.

Typical Formula (g/l)

• Enzymatic Digest of Casein: 5.0 g


• Lactose: 4.0 g


• Sodium Phosphate: 10.0 g


• Sodium Selenite: 4.0 g

The final pH is maintained at 7.0 ± 0.2 at 25°C, ensuring an optimal environment for microbial growth.

Principle of the Method

The enzymatic digest of casein provides a rich source of amino acids, nitrogen, carbon, vitamins, and minerals essential for the growth of microorganisms. Lactose acts as a fermentable carbohydrate, while sodium phosphate serves as a buffer to stabilize the pH. Sodium selenite is the selective agent that inhibits the growth of many species of Gram-positive and Gram-negative bacteria, including enterococci and coliforms, allowing effective isolation of the species of interest.

Applications of Selenite Broth

• Isolation and identification of Salmonella spp. in food samples.


• Microbiological analysis in clinical settings.


• Contamination testing in food products.

Advantages of Selenite Broth

• High specificity in the enrichment of pathogenic bacteria.


• Ease of use and preparation.


• Fast and reliable results for diagnosis and analysis.

Using Selenite Broth means ensuring high-quality microbiological analysis, essential for food safety and public health. Choose our Selenite Broth in a pack of 20 tubes of 10 ml for a professional and precise approach in your microbiological investigations.