The Steril Control GST E6 AMPOULES biological indicators are essential tools for ensuring the effectiveness of steam sterilization processes. These vials contain spores of Geobacillus stearothermophilus (ATCC® 7953), a highly resistant microorganism used to verify the functionality of autoclaves and sterilization cycles. Their use is fundamental in pharmaceutical, medical, and laboratory settings, where sterility is crucial for the safety of products and patients.

Product Features

• Regulatory Compliance: Complies with USP, EP, and DAB standards, ensuring high levels of quality and safety.


• Content: Each vial contains a specific nutrient broth, a pH indicator, and spores in predefined concentrations (E6=1-5x10⁶ CFU/vial; E5=1-5x10⁵ CFU/vial; E4=1-5x10⁴ CFU/vial).


• Efficiency: The spores are completely destroyed after 15 minutes of sterilization at 121°C, ensuring reliable results.

Instructions for Use

For proper use of Steril Control GST E6 AMPOULES biological indicators, follow these steps:

1. Remove one or more vials from the package.


2. Place the vials in the most problematic areas of the autoclave, such as the lower shelf and near the door.


3. Remove the vials after the sterilization cycle and incubate them at 55-60°C (131-140°F) for 24-48 hours.


4. Incubate one unsterilized vial as a positive control.


5. Evaluate the color of the vials to determine the effectiveness of sterilization.

Result Interpretation

Evaluating the results is simple and immediate:

• Purple/Clear Color: Indicates that the spores have been destroyed and sterilization was successful.


• Yellow/Turbid Color: Indicates microbial growth, suggesting that sterilization was not effective.

Treatment of Vials after Use

After use, it is essential to properly treat positive vials (yellow/turbid):

• Autoclave the vials at 121°C for at least 30 minutes.


• Dispose of the vials following standard laboratory practices.

Why Choose Steril Control GST E6 AMPOULES Biological Indicators?

These biological indicators offer a reliable and standardized method for monitoring the effectiveness of sterilization processes. Choosing Steril Control GST E6 AMPOULES means ensuring the safety and quality of sterilized products, thereby protecting patient health and the integrity of the materials used.