VP (KOH) Reagent Droppers

Reagents for the Voges-Proskauer test, in dropper bottles.

INTENDED USE
Reagent kit for performing the Voges-Proskauer test, used as a qualitative test to differentiate Enterobacteriaceae based on their ability to produce acetyl methyl carbinol (acetoin) by fermenting glucose. The test is intended as an aid to diagnosis, and additional tests are required to complete the diagnostic results.
DESCRIPTION
VP (KOH) Reagent Droppers is used for the identification of bacteria, in particular of the Enterobacteriaceae family, based on the production of acetylmethylcarbinol (acetoin) from glucose metabolism.
KIT CONTENTS
• 10 dropper bottles of sodium alpha naphthol (2.5 ml)
• 10 dropper bottles of potassium hydroxide (2.5 ml).
PRINCIPLE OF THE METHOD
Some bacteria, when cultured in specific media such as MR-VP broth, metabolize glucose to produce acetylmethylcarbinol. Upon addition of the Voges-Proskauer reagents to a culture tube containing MR-VP broth, any acetylmethylcarbinol present is oxidized to methyl diacetylcarbinol, which reacts with creatine (in the broth) and forms a red-colored compound.